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Supreme activity of gramicidin S towards resistant, persistent and biofilm cells of staphylococci and enterococci Marina BerditschWhile mitotic spindle defects bring on mitotic delays, many cells divide, at an incidence close to that of untreated cells. Aurora A inhibition results in chromosome congression defects at metaphase, lagging chromosomes
Subsequently, a 200-μl fixation buffer was accustomed to take care of the cells for twenty min. Following washing the cells three times with precooled PBS, one× intracellular staining permeabilization clean buffer made up of 1% DAPI (blue) was applied to mark the nuclei from the cells. Ten minutes afterwards, the cells were being washed thrice w
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